plasmid (Addgene inc)
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Addgene inc
plasmid
Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+mit+2mutaeq/pcDNA3%2E1%2B%2Fmit-2mutAEQ+(Plasmid+%2345539)/pm41559344-51-12-15
Average 93 stars, based on 9 article reviews
Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcdna3+1+mit+2mutaeq/pcDNA3%2E1%2B%2Fmit-2mutAEQ+(Plasmid+%2345539)/pm41559344-51-12-15
Average 93 stars, based on 9 article reviews
plasmid - by Bioz Stars,
2026-11
93/100 stars
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Purification:Article Title: Important Role of Sarcoplasmic Reticulum Ca 2+ Release via Ryanodine Receptor-2 Channel in Hypoxia-Induced Rieske Iron–Sulfur Protein-Mediated Mitochondrial Reactive Oxygen Species Generation in Pulmonary Artery Smooth Muscle Cells Article Snippet: Mitochondria-targeted Ca 2+ sensor pcDNA3.1+/mit-2mutAEQ (Asp119 to Ala, Asn28 to Leu points mutations on aequorin) was a gift from Javier Alvarez-Martin (plasmid No. 45539; Addgene, Watertown, MA). .. Following the procedure provided by Addgene ( 6 ), Transfection:Article Title: Important Role of Sarcoplasmic Reticulum Ca 2+ Release via Ryanodine Receptor-2 Channel in Hypoxia-Induced Rieske Iron–Sulfur Protein-Mediated Mitochondrial Reactive Oxygen Species Generation in Pulmonary Artery Smooth Muscle Cells Article Snippet: Mitochondria-targeted Ca 2+ sensor pcDNA3.1+/mit-2mutAEQ (Asp119 to Ala, Asn28 to Leu points mutations on aequorin) was a gift from Javier Alvarez-Martin (plasmid No. 45539; Addgene, Watertown, MA). .. Following the procedure provided by Addgene ( 6 ), Cell Culture:Article Title: Important Role of Sarcoplasmic Reticulum Ca 2+ Release via Ryanodine Receptor-2 Channel in Hypoxia-Induced Rieske Iron–Sulfur Protein-Mediated Mitochondrial Reactive Oxygen Species Generation in Pulmonary Artery Smooth Muscle Cells Article Snippet: Mitochondria-targeted Ca 2+ sensor pcDNA3.1+/mit-2mutAEQ (Asp119 to Ala, Asn28 to Leu points mutations on aequorin) was a gift from Javier Alvarez-Martin (plasmid No. 45539; Addgene, Watertown, MA). .. Following the procedure provided by Addgene ( 6 ), Incubation:Article Title: Important Role of Sarcoplasmic Reticulum Ca 2+ Release via Ryanodine Receptor-2 Channel in Hypoxia-Induced Rieske Iron–Sulfur Protein-Mediated Mitochondrial Reactive Oxygen Species Generation in Pulmonary Artery Smooth Muscle Cells Article Snippet: Mitochondria-targeted Ca 2+ sensor pcDNA3.1+/mit-2mutAEQ (Asp119 to Ala, Asn28 to Leu points mutations on aequorin) was a gift from Javier Alvarez-Martin (plasmid No. 45539; Addgene, Watertown, MA). .. Following the procedure provided by Addgene ( 6 ), Amplification:Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response Article Snippet: The target DNA for genome editing was prepared by annealing two DNA oligos and was ligated into a BbsI-digested sgRNA expression vector. .. Mitochondrial-targeted aequorin N26D was amplified from Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response. Article Snippet: The target DNA for genome editing was prepared by annealing two DNA oligos and was ligated into a BbsI-digested sgRNA expression vector. .. Mitochondrial-targeted aequorin N26D was amplified from Plasmid Preparation:Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response Article Snippet: The target DNA for genome editing was prepared by annealing two DNA oligos and was ligated into a BbsI-digested sgRNA expression vector. .. Mitochondrial-targeted aequorin N26D was amplified from Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response. Article Snippet: The target DNA for genome editing was prepared by annealing two DNA oligos and was ligated into a BbsI-digested sgRNA expression vector. .. Mitochondrial-targeted aequorin N26D was amplified from Polymerase Chain Reaction:Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response Article Snippet: The target DNA for genome editing was prepared by annealing two DNA oligos and was ligated into a BbsI-digested sgRNA expression vector. .. Mitochondrial-targeted aequorin N26D was amplified from Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response Article Snippet: Mitochondrial-targeted aequorin N26D was amplified from pcDNA3.1+/mit-2mutAEQ (Addgene plasmid #45539) by PCR with repairing two mutations, L28N and A119D, to the WT amino acids and was ligated to the pCAGGS vector. .. Ligation of PCR products into vectors was performed by the SLiCE method ( , ). Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response. Article Snippet: Mitochondrial-targeted aequorin N26D was amplified from pcDNA3.1+/mit-2mutAEQ (Addgene plasmid #45539) by PCR with repairing two mutations, L28N and A119D, to the WT amino acids and was ligated to the pCAGGS vector. .. Ligation of PCR products into vectors was performed by the SLiCE method (79, 80). Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response. Article Snippet: The target DNA for genome editing was prepared by annealing two DNA oligos and was ligated into a BbsI-digested sgRNA expression vector. .. Mitochondrial-targeted aequorin N26D was amplified from Ligation:Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response Article Snippet: Mitochondrial-targeted aequorin N26D was amplified from pcDNA3.1+/mit-2mutAEQ (Addgene plasmid #45539) by PCR with repairing two mutations, L28N and A119D, to the WT amino acids and was ligated to the pCAGGS vector. .. Ligation of PCR products into vectors was performed by the SLiCE method ( , ). Article Title: Mammalian type opsin 5 preferentially activates G14 in Gq-type G proteins triggering intracellular calcium response. Article Snippet: Mitochondrial-targeted aequorin N26D was amplified from pcDNA3.1+/mit-2mutAEQ (Addgene plasmid #45539) by PCR with repairing two mutations, L28N and A119D, to the WT amino acids and was ligated to the pCAGGS vector. .. Ligation of PCR products into vectors was performed by the SLiCE method (79, 80). Recombinant:Article Title: Optogenetic manipulation of Gq- and Gi/o-coupled receptor signaling in neurons and heart muscle cells Article Snippet: Recombinant DNA reagent , pGloSesor-20F cAMP , Promega , GeneBank: EU770615.1 , . .. Recombinant DNA reagent , Article Title: Coral anthozoan-specific opsins employ a novel chloride counterion for spectral tuning Article Snippet: Recombinant DNA reagent , pMT-Antho2c_1D4 , This study , , The CDS of Antho2e was tagged with rho 1D4 epitope and inserted into the multicloning site of pMT vector (see Materials and methods section). Available from Akihisa Terakita lab.. .. Recombinant DNA reagent , |